21 % GH₵920.40
Human Elisa Kit

Human Inhibin A (INH-A) ELISAKit

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  • Description
  • Expédition et retours
Assay range: 3ng/L-120ng/L
96 determinations
 
Purpose
This kit allows for the determination of INH-A concentrations in Human serum, cell culture supernates and other biological fluids
 
Principle
The kit assay Human INH-A level in the sample?use Purified Human INH-A antibody to coat microtiter plate wells. Make solid-phase antibody, then add INH-A to wells. Combined INH-A antibody which With HRP labeled,become antibody - antigen - enzyme-antibodycomplex. After washing Completely, add TMB substrate solution.TMB substrate becomes bluecolor At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human INH-A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 
Assay procedure
1. Dilute and add sample: Dilute Original density Standard as follows table:
2. Add sample: Set blank wells separately (blank comparison wells don’t add sample and HRP-Conjugate reagent; each step operation is the same). testing sample well. Add 50μl of the standard to the Microelisa strip plate, add Sample dilution 40μl to the testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells, don’t touch the well wall as far as possible, and Gently mix.
3. Incubate: After closing the plate with the Closure plate membrane, incubate for 30 min at 37.
4. Configurate liquid: 30-fold (or 20-fold) wash solution diluted 30-fold (or 20-fold) with distilled water and reserve.
5. Washing: Uncover the Closure plate membrane, discard the Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.
6. Add enzyme: Add HRP-Conjugate reagent 50μl to each well, except the blank well.
7. Incubate: Operation with 3.
8.Washing: Operation with 5.
9.Color: Add Chromogen Solution A 50ul and Chromogen Solution B 50ul to each well, evade the light preservation for 15 min at 37
10.Stop the reaction: Add Stop Solution 50μl to each well, Stop the reaction (the blue color change to yellow color).
11. Assay: take blank well as zero, Read absorbance at 450nm after Adding Stop Solution and within 15min.
 
Calculate
Take the standard density as the horizontal, the OD value for the vertical, draw the
the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density and the OD value, with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.
 
Important notes
1. The kit when taken out from the refrigeration environment should be balanced 15-30 minutes at room temperature, ELISA plates coated if not used up after opening, the plate should be stored in Sealed bag.
2. The washing buffer facilitates crystallization and separation. Heating the buffer helps dissolve the crystals when diluted. Washing does not affect the result.
3. Add a Sample with a sampler at Each step, and proofread its accuracy frequently, to avoid the experimental error. Add sample within 5 mins. If the number of sample is much, recommend to use Volley.
4. If the testing material content is excessively higher (The sample OD is bigger than the first standard well ), please dilute the Sample (n-fold). After dilution, multiply the result by the dilution factor to obtain the accurate concentration. 
5. Closure plate membrane only limits the disposable use, to avoid cross-contamination.
6. The substrate evades the light preservation.
7. Please according to the instructions for use, the test result determination must take the microtiter plate reader as a standard.
8. All samples, washing buffers and waste materials MUST be handled and disposed off according to the standard protocols for infectious materials.
9. Do not mix reagents with those from other lots.
 
Storage and validity 
1 Storage 2-8
2 validity six months

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