DNase I(Deoxyribonuelease?) 25mg

General Information

• Product Name: DNase I (Deoxyribonuclease I)
• Enzyme Commission (EC) Number: 3.1.21.1
• Source: Typically derived from Bovine Pancreas
• CAS Number: 9003-98-9
• Molecular Formula: Protein (Enzyme)
• Molecular Weight: ~31 kDa

Physical and Chemical Characteristics

• Appearance: White to off-white lyophilized powder
• Solubility: Soluble in water and standard biological buffers
• Optimum pH: 7.0-8.0
• Optimum Temperature: 37°C
• Cofactors: Requires divalent cations (Mg²? or Ca²?) for activity

Specifications

• Enzyme Activity: ≥2,000 Kunitz units/mg (1 Kunitz unit causes an increase in absorbance at 260 nm by 0.001 per minute at 25°C, pH 5.0)
• Purity: ≥90% by SDS-PAGE
• Endotoxin Level: ≤1 EU/mg
• Microbial Contamination: ≤100 CFU/g
• Heavy Metals: ≤10 ppm
• Moisture Content: ≤5.0%

Stability and Storage

• Stability: Stable for at least 2 years when stored properly
• Storage Conditions: Store at -20°C for long-term stability; protect from moisture and light
• Reconstitution Stability: Once reconstituted, store at 2-8°C and use within a few days

Handling and Safety

• Handling: Avoid direct contact; use in a well-ventilated area
• Safety Precautions: Wear protective gloves, safety glasses, and a dust mask when handling
• Disposal: Dispose of according to local environmental regulations and guidelines

Regulatory Compliance

• Biochemical Grade: Suitable for research and industrial applications
• Pharmaceutical Grade: Compliant with USP and EP standards (if applicable)
• ISO Certification: Compliant with International Organization for Standardization (ISO) standards

Packaging

• Packaging Size: 25mg
• Packaging Material: Airtight vials or sealed containers
• Labeling: Includes product name, enzyme activity, molecular weight, CAS number, and batch number

Applications

• Molecular Biology Research: Used for DNA degradation in molecular biology protocols
• Cell Culture Applications: Reduces extracellular DNA in cell culture media
• Biopharmaceuticals: Used in the purification of protein and nucleic acid preparations
• Diagnostic Applications: Applied in nucleic acid-based assays and forensic science