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- HEPES buffer,2×(pH7.2-7.4?sterile) HEPES buffer,2×(pH7.2-7.4?sterile)


HEPES buffer,2×(pH7.2-7.4?sterile)
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General Information
1. Product Name: HEPES buffer, 2× (pH 7.2-7.4, sterile)
2. Product Description: A sterile, 2× concentrated solution of HEPES (N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid)) buffer, adjusted to a pH range of 7.2-7.4.
Physical and Chemical Characteristics
1. Appearance: Clear, colorless solution
2. pH: 7.2-7.4
3. Concentration: 2× concentrated solution (approximately 100-120 mM)
4. Sterility: Sterile filtered or autoclaved
Specifications
1. Assay: ≥99% (HPLC)
2. pH: 7.2-7.4
3. Concentration: 100-120 mM
4. Endotoxin: ≤0.1 EU/mL
5. Sterility: Sterile filtered or autoclaved
6. Particulate Matter: ≤10 particles/mL (≥ 10 μm)
Composition
1. HEPES: 100-120 mM
2. Sodium Chloride (NaCl): 140-160 mM
3. Potassium Chloride (KCl): 5-10 mM
Applications
1. Cell Culture: Used as a buffering agent to maintain physiological pH in cell culture media.
2. Molecular Biology: Used as a buffering agent in various molecular biology applications, such as PCR, DNA sequencing, and protein purification.
3. Biochemistry: Used as a buffering agent in various biochemical applications, such as enzyme assays and protein stability studies.
Storage and Handling
1. Storage: Store at 2-8°C.
2. Handling: Handle with care, as the solution may be irritating to skin and eyes.
3. Shelf Life: 12 months from the date of manufacture.
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