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- STE/TNE Buffer STE/TNE Buffer
STE/TNE Buffer
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General Information
1. Product Name: STE/TNE Buffer
2. Product Description: A solution of Sodium Chloride, Tris-HCl, and EDTA (STE) or a solution of Tris-HCl, NaCl, and EDTA (TNE), used as a buffer in various molecular biology and biochemical applications.
Physical and Chemical Characteristics
1. Appearance: Clear, colorless solution
2. pH: 7.4-8.0
3. Concentration: Varies depending on specific formulation
4. Sterility: Sterile filtered or autoclaved
Specifications
1. Assay: ≥99% (HPLC)
2. pH: 7.4-8.0
3. Concentration: Varies depending on specific formulation
4. Endotoxin: ≤0.1 EU/mL
5. Sterility: Sterile filtered or autoclaved
6. Particulate Matter: ≤10 particles/mL (≥ 10 μm)
Composition
STE Buffer:
1. Sodium Chloride (NaCl): 100mM
2. Tris-HCl: 10mM
3. EDTA: 1mM
TNE Buffer:
1. Tris-HCl: 10mM
2. Sodium Chloride (NaCl): 100mM
3. EDTA: 1mM
Applications
1. Molecular Biology: Used as a buffer in various molecular biology applications, such as DNA extraction, PCR, and DNA sequencing.
2. Biochemistry: Used as a buffer in various biochemical applications, such as enzyme assays and protein purification.
3. Cell Culture: Used as a buffer in cell culture media to maintain physiological pH.
Storage and Handling
1. Storage: Store at 2-8°C.
2. Handling: Handle with care, as the solution may be irritating to skin and eyes.
3. Shelf Life: 12 months from the date of manufacture.
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