{"title":"Molecular Biology,next-gen sequencing","description":"\u003cp\u003eExplore our comprehensive \u003cstrong data-start=\"415\" data-end=\"436\"\u003eMolecular Biology\u003c\/strong\u003e product range designed to support every stage of genetic and biochemical research. From DNA\/RNA extraction kits and PCR reagents to enzymes, cloning tools, and electrophoresis supplies, our catalog includes everything needed for accurate and reproducible results. Trusted by researchers worldwide, our products are rigorously tested for purity, stability, and performance. Ideal for applications such as gene expression analysis, sequencing, CRISPR, qPCR, and protein studies. Whether you're in academia, biotech, or diagnostics, our molecular biology reagents help power your discoveries. Fast shipping and expert support available—shop now to streamline your workflow.\u003c\/p\u003e","products":[{"product_id":"silica-membrane-based-mini-spin-column","title":"Silica membrane based Mini spin column","description":"\u003cp\u003e\u003cstrong\u003eSilica membrane based Mini spin column\u003c\/strong\u003e is a high-quality laboratory product supplied by \u003cstrong\u003eGRSC Store\u003c\/strong\u003e for academic and institutional research in \u003cstrong\u003eGhana\u003c\/strong\u003e. This product is intended for use in university laboratories, research institutions, and educational facilities.\u003c\/p\u003e\u003cdiv\u003e\n\u003ch3\u003e\n\u003cstrong\u003eFeedback from one of our customers\u003c\/strong\u003e:\u003c\/h3\u003e\r\n\u003cp\u003eHere are the observations that I made about your columns:\u003c\/p\u003e\r\n\u003cp\u003e1) Based on the ratio of RNA-to-DNA extraction your columns actually turned out to be somewhat suboptimal, they end up with capturing a bit too much DNA together with the RNA\u003c\/p\u003e\r\n\u003cp\u003e2) However, the total amount of extracted nucleic acid product is very high. Based on this I am expecting your columns to be optimal for DNA extraction\u003c\/p\u003e\r\n\u003cp\u003e3) Visually the columns look very nice. I have observed that even when using ThermoFisher Kit columns, I see some settling of the membrane at the end of all steps (that is due to centrifugation the membrane compresses and there is a visible separation between the membrane and the o-ring that holds it in place). However, this effect was almost completely absent from your columns, so the craftmanship and quality of the membrane look very good.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003col\u003e\r\n\u003cli\u003eSilica membrane-based mini spin column for DNA\/RNA extraction;\u003c\/li\u003e\r\n\u003cli\u003e8 layers advanced membranes for 100% quality guaranteed;\u003c\/li\u003e\r\n\u003cli\u003eHigh quality for DNA\/RNA purification;\u003c\/li\u003e\r\n\u003cli\u003eUnique flower-O-ring washer design ensuring no liquid residues;\u003c\/li\u003e\r\n\u003cli\u003eMost popular mini spin columns in UAS and Canada marke\u003c\/li\u003e\r\n\u003c\/ol\u003e\n\u003c\/div\u003e\u003cp\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e Analytical and experimental workflows in biochemistry, molecular biology, and laboratory research. Please refer to product-specific datasheets for detailed handling and safety information.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSupplier:\u003c\/strong\u003e GRSC Store — reliable delivery across Ghana for institutions and labs.\u003c\/p\u003e","brand":"Molecular Biology","offers":[{"title":"Default Title","offer_id":46748319252706,"sku":null,"price":27.6,"currency_code":"GHS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0756\/4070\/1154\/files\/S1910.webp?v=1778948863"},{"product_id":"rnafixer-stabilization-solution","title":"RNAfixer Stabilization Solution","description":"\u003cp\u003e\u003cstrong\u003eRNAfixer Stabilization Solution\u003c\/strong\u003e is a high-quality laboratory product supplied by \u003cstrong\u003eGRSC Store\u003c\/strong\u003e for academic and institutional research in \u003cstrong\u003eGhana\u003c\/strong\u003e. This product is intended for use in university laboratories, research institutions, and educational facilities.\u003c\/p\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Storage and Stability:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe transparent liquid can be stored at room temperature (18-25°C) for 12 months. If precipitation or separation is observed when using the product, it can be heated at 37°C to dissolve it before use, without affecting the quality of the product.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Introduction:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eSuitable for animal tissues (heart, liver, kidney, muscle, testis, brain, spleen, etc.), cultured cells, RNA viruses, fruit flies, bacteria, leukocytes, whole blood, and some plant tissues, etc.\u003c\/p\u003e\n\u003cp\u003eRNAfixer is an aqueous and non-toxic tissue preservation solution that can quickly penetrate the cytoplasm of fresh tissue cells, stabilizing and protecting the RNA in situ in a non-frozen state. Immersion of tissue sections in RNAfixer immediately after removal does not affect the quality and quantity of RNA extraction in the future. RNAfixer eliminates the inconvenience of immediate processing or liquid nitrogen preservation of RNA samples. After immersion in RNAfixer, RNA in fresh tissue cells can be well preserved at 37°C for one day, one week at 25°C, one month at 4°C, and long-term preservation at -20°C or -80°C. 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It can also be used directly for tissue sections, immunology, and flow cytometry analysis without affecting the quality of RNA extraction.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/div\u003e\u003cp\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e Analytical and experimental workflows in biochemistry, molecular biology, and laboratory research. Please refer to product-specific datasheets for detailed handling and safety information.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSupplier:\u003c\/strong\u003e GRSC Store — reliable delivery across Ghana for institutions and labs.\u003c\/p\u003e","brand":"Molecular Biology","offers":[{"title":"Default Title","offer_id":46748320858338,"sku":null,"price":2268.0,"currency_code":"GHS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0756\/4070\/1154\/files\/rna-mini-kit-for-tissues-and-cells-rna.jpg?v=1778949147"},{"product_id":"fasthifi-pcr-master-mix","title":"FastHiFi PCR Master Mix","description":"\u003cp\u003e\u003cstrong\u003eFastHiFi PCR Master Mix\u003c\/strong\u003e is a high-quality laboratory product supplied by \u003cstrong\u003eGRSC Store\u003c\/strong\u003e for academic and institutional research in \u003cstrong\u003eGhana\u003c\/strong\u003e. This product is intended for use in university laboratories, research institutions, and educational facilities.\u003c\/p\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cstrong\u003eStore: \u003c\/strong\u003e20°C, 2 years\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe kit contains CYA8 FastHiFi DNA Polymerase, dNTPs and optimized reaction buffer at a concentration of 2x. Before reaction, DNA\/cDNA template, specific primer, and ddH2O should be prepared to mix the final concentration of 1×.\u003c\/p\u003e\n\u003cp\u003eA8 is from the genetic engineering modification Pfu, which can greatly improve the amplification rate, fidelity and yield. So A8 Mix is the preferred product for non-long fragments ultra-fidelity fast amplification.\u003c\/p\u003e\n\u003cp\u003eThe Mix contains red tracer dye for quicker electrophoresis. And the PCR amplification products are with blunt end, which can be directly employed to the clone reaction with our pTOPO vector. Of course, after purified, the amplification products also can be used for other follow-up experiment, such as digestion, ligation and fluorescence sequencing.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Features\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1.Fast amplification: 2-4kb\/min, up to 4-8 times of Pfu;\u003c\/p\u003e\n\u003cp\u003e2.High Yield: Up to 1.5-2 times of traditional Pfu;\u003c\/p\u003e\n\u003cp\u003e3.Super Fidelity: Up to 54 times of traditional Taq;\u003c\/p\u003e\n\u003cp\u003e4.With complex genomic DNA as template for the amplification of not more than 3kb of the product, with simple genome, plasmid and phage DNA as a template for the amplification of not more than 6kb of the product.\u003c\/p\u003e\n\u003c\/div\u003e\u003cp\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e Analytical and experimental workflows in biochemistry, molecular biology, and laboratory research. 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This product is intended for use in university laboratories, research institutions, and educational facilities.\u003c\/p\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cstrong\u003eStorage and Stability\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003cp\u003e2 x SYBR Green qPCR Mix should be stored protected from light at -20°C for long term storage (more than 6 months), or at 4°C for short term storage. Avoid repeated freezing and thawing.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003cp\u003e2 x SYBR Green qPCR Mix provides rapid real-time quantification of RNA targets in SYBR Green fluorescent. The components of 2 x SYBR Green qPCR Mix include Hot-start enzyme named HotMaster Taq DNA Polymerase, dNTP, PCR reaction buffer, BSA and SYBR Green, and so on. The fluorescent dye SYBR Green I in the master mix enables the analysis of many different targets without having to synthesize target specific labeled probes. High specificity and sensitivity in PCR are achieved by the use of the hot-start enzyme HotMaster Taq DNA Polymerase together with a specialized PCR buffer.\u003c\/p\u003e\r\n\u003cp\u003eUnlike other “hot-start” Taq DNA polymerase formulations that block the enzyme activity only in the first high temperature step, HotMaster Taq DNA inhibitors closes the polymerase-substrate binding site by temperature adjustment method. Inactive polymerase-inhibitor complexes are formed at temperatures below 40°C. As the temperature is elevated to the primer-specific annealing temperature, the binding equilibrium is shifted towards complex formation only with target-specific primed template DNA. This prevents the formation of mis-primed products and primer–dimers during reaction setup and the first denaturation step, leading to high PCR specificity and accurate quantification.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003col\u003e\r\n\u003cli\u003eReference Dye ROX is not included in 2 x SYBR Green qPCR Mix. It is for the researcher to decide whether or not ROX reference dye should be included in the experiment, on the basis of technical protocol of qPCR instruments. Its aim is to eliminate the background signal and to adjust and compensate lot-to-lot fluorescence signal error. The catalog number of the supporting ROX product is PC38 ROX reference dye.\u003c\/li\u003e\r\n\u003cli\u003eSYBR® Green I dye is light sensitive and should be stored in the dark, and be protected from light when use.\u003c\/li\u003e\r\n\u003cli\u003eWe strongly recommend preparing the PCR reaction on ice, for higher specificity and less background.\u003c\/li\u003e\r\n\u003cli\u003eMg2+ concentration in 2 x SYBR Green qPCR Mix is 4 mM. We strongly recommend an initial Mg2+ concentration of 2mM. Proper Mg2+ concentration can be achieved by using 25mM MgCl2.\u003c\/li\u003e\r\n\u003c\/ol\u003e\n\u003c\/div\u003e\u003cp\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e Analytical and experimental workflows in biochemistry, molecular biology, and laboratory research. 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This product is intended for use in university laboratories, research institutions, and educational facilities.\u003c\/p\u003e\u003cdiv\u003e\n\u003ch3\u003eNote: All reagents, when store at indicated temperature, are stable for 9 months.\u003c\/h3\u003e\r\n\u003cp\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003cp\u003eThe innovative CHANGYU plus system is ideal for the rapid purification of genomic DNA-free total RNA from animal cells or tissues. The unique genomic DNA elimination columns effectively remove genomic DNA from RNA samples. No DNase digestion step is required. The purified total RNA can be directly used for reverse transcription and RT-PCR. The whole process is phenol\/chloroform-free. The unique lysis buffer immediately lyses biological samples and inactivates RNase and DNase. The lysate containing RNA is then passed through a genomic DNA elimination column, where genomic DNA binds to the column membrane, and RNA remains in the flow-through. Ethanol is added to the flow-through to provide appropriate binding conditions for RNA, and RNA selectively binds to the silica membrane of the RNA column in the high-salt buffer. RNA is purified through a series of wash-spin steps to remove protein followed by elution of RNA from silica-membrane with RNase-free H\u003csub\u003e2\u003c\/sub\u003eO.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003col\u003e\r\n\u003cli\u003eUnique gDNA Elimination columns avoid the need for DNase\u003c\/li\u003e\r\n\u003cli\u003eEfficient removal of genomic DNA\u003c\/li\u003e\r\n\u003cli\u003eHighly reproducible yields of RNA in minutes\u003c\/li\u003e\r\n\u003cli\u003eHigh-performance RNA for sensitive applications\u003c\/li\u003e\r\n\u003c\/ol\u003e\n\u003c\/div\u003e\u003cp\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e Analytical and experimental workflows in biochemistry, molecular biology, and laboratory research. 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This product is intended for use in university laboratories, research institutions, and educational facilities.\u003c\/p\u003e\u003cdiv\u003e\n\u003ch3\u003eNote: All reagents, when store at -20 °C, are stable for 12 months.\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe Zero Background TA Simple Topoisomerase \u003cstrong\u003eCloning Kit\u003c\/strong\u003e is designed for the fast cloning of DNA fragments up to 10 kb generated by Taq DNA polymerase, which introduces a single 3’-A overhang to the end of the DNA product. DNA fragments generated by restriction digestion or mechanical shearing can also be effectively cloned after end polishing using Taq DNA polymerase. It utilizes the DNA strand transfer activity of Vaccinia virus topoisomerase I. Vaccinia virus DNA topoisomerase I form a 3’-phosphoryl intermediate with the plasmid vector containing a cleavage recognition motif of 5’CCCTT↓. Covalently bound topoisomerase I then transfer the incised vector DNA strand to the DNA fragment to be cloned with free 5’-OH terminuses. This transferring reaction is rapid and reproducible. This kit’s cloning vector \u003cstrong\u003epTOPO-TA Simple\u003c\/strong\u003e is a high-copy number plasmid engineered to tolerate mild toxic genes. Regions flanking the cloning site of pTOPO-TA Simple vectors do not contain any common restriction sites, eliminating the possible redundancy of restriction sites.\u003c\/p\u003e\n\u003c\/div\u003e\u003cp\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e Analytical and experimental workflows in biochemistry, molecular biology, and laboratory research. 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This product is intended for use in university laboratories, research institutions, and educational facilities.\u003c\/p\u003e\u003cdiv\u003e\n\u003cp\u003eAll reagents, when stored in indicated temperature, are stable for 12 months.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eReagents required, but not supplied:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003col\u003e\n\u003cli\u003eChloroform\u003c\/li\u003e\n\u003cli\u003eIsopropyl alcohol\u003c\/li\u003e\n\u003cli\u003e75% Ethanol (in RNA-free water)\u003c\/li\u003e\n\u003cli\u003eRNase-free water or 0.5% SDS solution [To prepare RNase-free water, draw water into RNase-free glass bottles. Add diethylpyrocarbonate (DEPC) to 0.01% (v\/v). Let stand overnight and autoclave. The SDS solution must be prepared using DEPC-treated, autoclaved water.]\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003cstrong\u003eWARNING:\u003c\/strong\u003e Toxic in contact with skin and if swallowed. Causes burns. After contact with skin, wash immediately with plenty of detergent and water. If you feel unwell, seek medical advice (show label where possible). Phenol (108-95-2) and other Components (NJTSRN 80100437-5000p).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eTRIpure Reagent is a ready-to-use reagent for the isolation of total RNA from cells and tissues. The reagent, a mono-phasic solution of phenol and guanidine isothiocyanate, is an improvement to the single-step RNA isolation method. During sample homogenization or lysis, TRIpure Reagent maintains the integrity of the RNA, while disrupting cells and dissolving cell components.\u003c\/p\u003e\n\u003cp\u003eAddition of chloroform followed by centrifugation, separates the solution into an aqueous phase and an organic phase. RNA remains exclusively in the aqueous phase. After transfer of the aqueous phase, the RNA is recovered by precipitation with isopropyl alcohol. After removal of the aqueous phase, the DNA and proteins in the sample can be recovered by sequential precipitation. Precipitation with ethanol yields DNA from the interphase, and an additional precipitation with isopropyl alcohol yields proteins from the organic phase. Copurification of the DNA may be useful for normalizing RNA yields from sample to sample.\u003c\/p\u003e\n\u003cp\u003eThis technique performs well with small quantities of tissue (50-100 mg) and cells (5 × 10\u003csup\u003e6\u003c\/sup\u003e), and large quantities of tissue (≥1 g) and cells (\u0026gt;10\u003csup\u003e7\u003c\/sup\u003e), of human, animal, plant, or bacterial origin.\u003c\/p\u003e\n\u003cp\u003eThe simplicity of the TRIpure Reagent method allows simultaneous processing of a large number of samples. The entire procedure can be completed in one hour. Total RNA isolated by TRIpure Reagent is free of protein and DNA contamination. It can be used for Northern blot analysis, dot blot hybridization, poly (A)+ selection, in vitro translation, RNase protection assay, and molecular cloning. For use in the polymerase chain reaction (PCR), treatment of the isolated RNA with amplification grade DNase I is recommended when the two primers lie within a single exon.\u003c\/p\u003e\n\u003c\/div\u003e\u003cp\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e Analytical and experimental workflows in biochemistry, molecular biology, and laboratory research. Please refer to product-specific datasheets for detailed handling and safety information.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSupplier:\u003c\/strong\u003e GRSC Store — reliable delivery across Ghana for institutions and labs.\u003c\/p\u003e","brand":"Molecular Biology","offers":[{"title":"Default Title","offer_id":46748410577122,"sku":null,"price":1800.0,"currency_code":"GHS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0756\/4070\/1154\/files\/tripure-reagent-same-as-invitrogens-trizol_1e774882-cdaf-4b21-8ff2-b222ab912570.jpg?v=1756152229"},{"product_id":"2xtaq-pcr-mastermix","title":"2xTaq PCR Mastermix","description":"\u003cp\u003e\u003cstrong\u003e2xTaq PCR Mastermix\u003c\/strong\u003e is a high-quality laboratory product supplied by \u003cstrong\u003eGRSC Store\u003c\/strong\u003e for academic and institutional research in \u003cstrong\u003eGhana\u003c\/strong\u003e. This product is intended for use in university laboratories, research institutions, and educational facilities.\u003c\/p\u003e\u003cdiv\u003e\n\u003cp\u003e\u003cstrong\u003eStorage: 2-8\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAttention:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eThis product contains phenol red, which may change color from orange to purple. \u003cstrong\u003e\u003cu\u003eColor change does not affect product quality or use.\u003c\/u\u003e\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Description:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eThis product contains Taq DNA polymerase, dNTPs, MgCl\u003csub\u003e2\u003c\/sub\u003e, and reaction buffer at a concentration of 2×. It has the advantages of being fast, easy, sensitive, specific, stable, and can minimize human errors. Only DNA template and primers are needed for use.\u003c\/li\u003e\n\u003cli\u003eThis product is convenient and easy to use, and can avoid contamination during PCR operation. It only needs to add an appropriate amount of 2×Taq PCR MasterMix solution, add the template and primers, and add deionized water to make the MasterMix solution concentration 1× for the reaction. Please ensure that it is fully dissolved and mixed before use, and the operation should be carried out on ice.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Contents:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eTaq DNA polymerase (recombinant): 0.1 units\/µl; MgCl\u003csub\u003e2\u003c\/sub\u003e: 4 mM; dNTPs (dATP, dCTP, dGTP, dTTP): 0.4 mM\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eQuality Control:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eNo exogenous nuclease activity was detected; it can effectively amplify single-copy genes in the human gene; no significant activity change was observed after one week of storage at room temperature.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eScope of Application:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eGene detection: This product has small differences between batches, especially suitable for large-scale gene detection, semi-quantitative PCR experiments, and the detection of trace DNA.\u003c\/li\u003e\n\u003cli\u003eAmplification of DNA and some complex templates with special structures and high GC content (\u0026gt;60%) and secondary structures: amplification of DNA fragments, DNA labeling, primer extension, and sequence determination. PCR products are A-tailed and can be directly cloned into T\/A vectors after purification.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\u003cp\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e Analytical and experimental workflows in biochemistry, molecular biology, and laboratory research. Please refer to product-specific datasheets for detailed handling and safety information.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSupplier:\u003c\/strong\u003e GRSC Store — reliable delivery across Ghana for institutions and labs.\u003c\/p\u003e","brand":"Molecular Biology","offers":[{"title":"Default Title","offer_id":46748422668514,"sku":null,"price":660.0,"currency_code":"GHS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0756\/4070\/1154\/files\/2xtaq-pcr-mastermix_7b159651-c58c-46f1-9823-866be23ad96a.jpg?v=1756152201"},{"product_id":"ptopo-blunt-cloning-kit","title":"pTOPO-Blunt Cloning Kit","description":"\u003cp\u003e\u003cstrong\u003epTOPO-Blunt Cloning Kit\u003c\/strong\u003e is a high-quality laboratory product supplied by \u003cstrong\u003eGRSC Store\u003c\/strong\u003e for academic and institutional research in \u003cstrong\u003eGhana\u003c\/strong\u003e. This product is intended for use in university laboratories, research institutions, and educational facilities.\u003c\/p\u003e\u003cdiv\u003e\n\u003ch3\u003eNote: All reagents, when store at -20 °C, are stable for 12 months.\u003c\/h3\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003cp\u003eThe Zero Background Blunt Topoisomerase \u003cstrong\u003eCloning Kit\u003c\/strong\u003e is designed to fast-cover blunt-ended DNA fragments up to 10 kb generated by high-fidelity DNA polymerases such as KOD, Pfu and Phusion. DNA fragments obtained by restriction digestion or mechanical shearing can also be cloned after end polishing to become blunt-ended. It utilizes the DNA strand transfer activity of Vaccinia virus topoisomerase I. Vaccinia virus DNA topoisomerase I form a 3’-phosphoryl intermediate with the plasmid vector containing a cleavage recognition motif of 5’CCCTT↓. Covalently bound topoisomerase I then transfer the incised vector DNA strand to the DNA fragment to be cloned with free 5’-OH terminuses. This transferring reaction is rapid and reproducible. The cloning vector pTOPO-Blunt in this kit are high-copy number plasmids engineered to tolerate mild toxic genes. Regions flanking the cloning site of pTOPO-Blunt vectors have multiple common restriction sites for releasing the cloned fragment by single or double restriction digestion.\u003c\/p\u003e\n\u003c\/div\u003e\u003cp\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e Analytical and experimental workflows in biochemistry, molecular biology, and laboratory research. 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