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- DNase I(Deoxyribonuelease?) 25mg DNase I(Deoxyribonuelease?) 25mg
DNase I(Deoxyribonuelease?) 25mg
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General Information
- Product Name: DNase I (Deoxyribonuclease I)
- Enzyme Commission (EC) Number: 3.1.21.1
- Source: Typically derived from Bovine Pancreas
- CAS Number: 9003-98-9
- Molecular Formula: Protein (Enzyme)
- Molecular Weight: ~31 kDa
Physical and Chemical Characteristics
- Appearance: White to off-white lyophilized powder
- Solubility: Soluble in water and standard biological buffers
- Optimum pH: 7.0-8.0
- Optimum Temperature: 37°C
- Cofactors: Requires divalent cations (Mg²? or Ca²?) for activity
Specifications
- Enzyme Activity: ≥2,000 Kunitz units/mg (1 Kunitz unit causes an increase in absorbance at 260 nm by 0.001 per minute at 25°C, pH 5.0)
- Purity: ≥90% by SDS-PAGE
- Endotoxin Level: ≤1 EU/mg
- Microbial Contamination: ≤100 CFU/g
- Heavy Metals: ≤10 ppm
- Moisture Content: ≤5.0%
Stability and Storage
- Stability: Stable for at least 2 years when stored properly
- Storage Conditions: Store at -20°C for long-term stability; protect from moisture and light
- Reconstitution Stability: Once reconstituted, store at 2-8°C and use within a few days
Handling and Safety
- Handling: Avoid direct contact; use in a well-ventilated area
- Safety Precautions: Wear protective gloves, safety glasses, and a dust mask when handling
- Disposal: Dispose of according to local environmental regulations and guidelines
Regulatory Compliance
- Biochemical Grade: Suitable for research and industrial applications
- Pharmaceutical Grade: Compliant with USP and EP standards (if applicable)
- ISO Certification: Compliant with International Organization for Standardization (ISO) standards
Packaging
- Packaging Size: 25mg
- Packaging Material: Airtight vials or sealed containers
- Labeling: Includes product name, enzyme activity, molecular weight, CAS number, and batch number
Applications
- Molecular Biology Research: Used for DNA degradation in molecular biology protocols
- Cell Culture Applications: Reduces extracellular DNA in cell culture media
- Biopharmaceuticals: Used in the purification of protein and nucleic acid preparations
- Diagnostic Applications: Applied in nucleic acid-based assays and forensic science
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